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Glucosidase IIβ (K117) polyclonal antibody（KU）

货号: BS2646

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2646

Product Name :Glucosidase IIβ (K117) polyclonal antibody（KU）

Swiss-Prot :P14314

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ). The α and β subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIα is the catalyitc core of the enzyme and can function independently of the β subunit. The sequence of Glucosidase IIβ encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIβ is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIβ was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIβ retains the complete complex in the ER.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Glucosidase IIβ (K117) polyclonal antibody detects endogenous levels of Glucosidase IIβ protein.

BiowMW :~ 59, 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Glucosidase 2 subunit beta; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain; PKCSH; PRKCSH; G19P1

Immunogen :Synthetic peptide, corresponding to amino acids 90-130 of Human Glucosidase IIβ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Glucosidase IIβ (K117) pAb at 1:500 dilution Lane1:The Testis tissue lysate of Mouse(40ug) Lane2:The Testis tissue lysate of Rat(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug) Lane6:MCF-7 whole cell lysate(40ug)