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EDG-5 (L303) polyclonal antibody

货号: BS2594

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2594

Product Name :EDG-5 (L303) polyclonal antibody

Swiss-Prot :O95136

Host :Rabbit

Reactivity :Human,Rat

Applications :IHC IF

Application_all :IHC: 1:50~1:200 IF: 1:50~1:200

Background :The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain. EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :EDG-5 (L303) polyclonal antibody detects endogenous levels of EDG-5 protein.

BiowMW :~ 39 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Sphingosine 1-phosphate receptor 2; S1P receptor 2; S1P2; S1P receptor Edg-5; S1PR2; EDG5

Immunogen :Synthetic peptide, corresponding to amino acids 281-330 of Human EDG-5.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of EDG-5 (L303) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of EDG-5 (L303) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Sphingosine‑1‑phosphate induces islet β‑cell proliferation and decreases cell apoptosis in high‑fat diet/streptozotocin diabetic mice

JournalExperimental and Therapeutic Medicine

ApplicationIHC

IF1.785

SpeciesMouse

PMID31602216