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DNMT3B (H7) polyclonal antibody

货号: BS2572

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2572

Product Name :DNMT3B (H7) polyclonal antibody

Swiss-Prot :Q9UBC3

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB:1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Dnmt3b (H7) polyclonal antibody detects endogenous levels of Dnmt3b protein.

BiowMW :~ 110 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :DNA Methylation,

Alternative Name :Dnmt3b; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; M.HsaIIIB

Immunogen :Synthetic peptide, corresponding to the N-terminual of Human DNMT3B.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of DNMT3B (H7) pAb in paraffin-embedded liver cancer tissue at 1:100.

Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions

JournalEuropean Journal Of Nutrition

ApplicationWB

IF4.449

SpeciesPig

PMID25410747
Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB

IF3.144

SpeciesMouse

PMID27592453