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Elongin A1 (R129) polyclonal antibody（KU）

货号: BS2530

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2530

Product Name :Elongin A1 (R129) polyclonal antibody（KU）

Swiss-Prot :Q14241

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Individuals harboring germline mutations in the tumor suppressor gene von Hippel-Lindau (VHL) exhibit an increased susceptibility to a variety of tumors including renal carcinoma, hemangio-blastoma of the central nervous system and pheochromo-cytoma. The Elongin (SIII) complex has been identified as the functional target of the VHL protein. Elongin (SIII) is a heterotrimer composed of a transcriptional active subunit designated Elongin A and two regulatory subunits designated Elongin B and Elongin C. VHL functions by binding to the Elongin B and C subunits, inhibiting the transcriptional efficacy of the Elongin (SIII) complex. The VHL protein migrates with an apparent molecular weight of 38 kDa. The Elongin A subunit is 773 amino acids in length and has an apparent molecular weight of 110 kDa, while Elongin B and C are 18 kDa and 15 kDa proteins, respectively.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Elongin A1 (R129) polyclonal antibody detects endogenous levels of Elongin A1 protein.

BiowMW :~ 100 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Transcription elongation factor B polypeptide 3; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Elongin A; EloA; Elongin 110 kDa subunit; TCEB3;

Immunogen :Synthetic peptide, corresponding to amino acids 98-142 of Human Elongin A1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Elongin A1 (R129) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Elongin A1 (R129) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.