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Hrs (R481) polyclonal antibody（KU）

货号: BS2448

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2448

Product Name :Hrs (R481) polyclonal antibody（KU）

Swiss-Prot :O14964

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a zinc-finger protein that interacts with STAM and undergoes tyrosine phosphorylation in response to IL2, CSF2 or HGF. Hrs is involved in intracellular trafficking and signal transduction and is associated with early endosomes. H contains a phosphatidylinositol 3-phosphate-binding domain that contributes to its endosomal targeting, where Hrs colocalizes with Clathrin via a Clathrin box motif at the carboxy terminus of Hrs. Hrs is essential for ventral folding morphogenesis and shares structual similarity to the yeast protein Vps27p, which is involved in vacuolar protein sorting. The human Hrs gene, which maps to chromosome 17q25, enodes a 777 amino acid protein. In Schwann cells, Hrs colocalizes at endosomes with the tumor suppressor protein schwannomin, suggesting a role for schwannomin in Hrs-mediated cell signaling.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Hrs (R481) polyclonal antibody detects endogenous levels of Hrs protein.

BiowMW :~ 86 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110; HGS; HRS

Immunogen :Synthetic peptide, corresponding to amino acids 461-510 of Human Hrs.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Hrs (R481) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of Hrs (R481) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing Cytoplasm and Nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Hrs (R481) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing Cytoplasm and Nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.