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Dbf4 (I18) polyclonal antibody（KU）

货号: BS2372

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2372

Product Name :Dbf4 (I18) polyclonal antibody（KU）

Swiss-Prot :Q9UBU7

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Dbf4 (I18) polyclonal antibody detects endogenous levels of Dbf4 (I18) protein.

BiowMW :~ 77 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein DBF4 homolog A; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1; DBF4; ASK; DBF4A; ZDBF1

Immunogen :Synthetic peptide, corresponding to the N-terminual of Human Dbf4.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Dbf4 (I18) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:The Testis tissue lysate of Mouse(40ug) Lane4:The Testis tissue lysate of Rat(40ug)