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DGK-ε (S194) polyclonal antibody

货号: BS2281

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2281

Product Name :DGK-ε (S194) polyclonal antibody

Swiss-Prot :P52429

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :IHC

Application_all :IHC: 1:50~1:200

Background :Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-i (130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :DGK-ε (S194) polyclonal antibody detects endogenous levels of DGK-ε protein.

BiowMW :~ 64 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGK-epsilon; DGKE; DAGK5

Immunogen :Synthetic peptide, corresponding to amino acids 180-230 of Human DGK-ε.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of DGK-ε(S194) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.