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Actinin-α3 (E25) polyclonal antibody（KU）

货号: BS2205

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS2205

Product Name :Actinin-α3 (E25) polyclonal antibody（KU）

Swiss-Prot :Q08043

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:500~1:1000 IF: 1:50~1:200

Background :The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Actinin-α3 (E25) polyclonal antibody detects endogenous levels of actinin-α3 protein, this antibody does not cross-react with other α-actinin isoforms.

BiowMW :~ 103 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; α-Actinin3; Actinin α3; Actininα3; αActinin-3; Actinin-α3; α-actinin-3

Immunogen :Synthetic peptide, corresponding to the N-terminus of Human Actinin-α3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Actinin-α3 (E25) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse muscle tissue lysate Lane3:Rat muscel tissue lysate

Immunohistochemistry (IHC) analyzes of Actinin-α3 (E25) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Actinin-α3 (E25) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.