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14-3-3 σ (E66) polyclonal antibody

货号: BS2178

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2178

Product Name :14-3-3 σ (E66) polyclonal antibody

Swiss-Prot :P31947

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :14-3-3 σ (E66) polyclonal antibody detects endogenous levels of 14-3-3 σ protein

BiowMW :~ 30 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin; SFN; HME1; YWHAS

Immunogen :Synthetic peptide, corresponding to amino acids 40-90 of Human 14-3-3 σ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of p16 INK4a expression in HEK293T (A), HeLa (B), mouse brain (C), rat brain (D) whole cell lysates.

Immunohistochemistry (IHC) analyzes of 14-3-3 σ (E66) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of 14-3-3 σ (E66) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.