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AATF (N16) polyclonal antibody（KU）

货号: BS2152

价格：￥ 660

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2152

Product Name :AATF (N16) polyclonal antibody（KU）

Swiss-Prot :Q9NY61

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Che-1 (protein AATF, apoptosis-antagonizing transcription factor) is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, Che-1 may function as a general inhibitor of the histone deacetylase HDAC1. Che-1 binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of Che-1. It also antagonizes PAR4 (prostate apoptosis response 4) mediated induction of aberrant amyloid peptide production in Alzheimer disease (AD; presenile and senile dementia). PAR4 is a leucine zipper protein that is pro-apoptotic and associated with neuronal degeneration in AD. Che-1 interaction with PAR4 suggests that it might directly or indirectly participate in regulation of PAR4 activity. Che-1 also co-localizes with PAR4 in both cytoplasmic and nuclear compartments, and it interacts directly and selectively with PAR4 via the leucine zipper domain in neural cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AATF (N16) polyclonal antibody detects endogenous levels of AATF protein.

BiowMW :~ 63 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein AATF; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; AATF; CHE1; DED; HSPC277

Immunogen :Synthetic peptide, corresponding to amino acids 10-60 of Human AATF.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AATF (N16) polyclonal antibody at 1:500 dilution Lane1:H1792 whole cell lysate(20ug) Lane2:HEK293T whole cell lysate(20ug) Lane3:PC12 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug)