产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

IARS2 (Y97) polyclonal antibody（KU）

货号: BS2148

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS2148

Product Name :IARS2 (Y97) polyclonal antibody（KU）

Swiss-Prot :Q9NSE4

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Accurate discrimination of the structurally similar amino acids valine and isoleucine by isoleucyl-tRNA synthetase (IleRS) results, in part, from a hydrolytic editing reaction, which prevents misactivated valine from being stably joined to tRNAIle. IleRS joins Ile to tRNA(Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. A member of the aminoacyl-tRNA synthetase family, human IleRS has been identified as a target of antibodies in the autoimmune disease polymyositis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IleRS (Y97) polyclonal antibody detects endogenous levels of IleRS protein.

BiowMW :~ 114 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Isoleucine--tRNA ligase, mitochondrial; Isoleucyl-tRNA synthetase; IleRS; IleRS

Immunogen :Synthetic peptide, corresponding to amino acids 67-127 of Human IleRS.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IleRS (Y97) polyclonal antibody at 1:1000 dilution Lane1:The Kidney tissue lysate of Mouse(40ug) Lane2:The Testis tissue lysate of Rat(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:Panc1 whole cell lysate(40ug)