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DGK-δ (S66) polyclonal antibody（KU）

货号: BS2078

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2078

Product Name :DGK-δ (S66) polyclonal antibody（KU）

Swiss-Prot :Q16760

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-α influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-β is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-γ encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-δ is most abundant in skeletal muscle. DGK-ε shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis. DGK-θ is most abundant in the cerebellum and hippocampus. DGK-ι is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb. DGK-η is closely related to DGK-δ. DGK-ζ is most abundant in brain and muscle. DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :DGK-δ (S66) polyclonal antibody detects endogenous levels of DGK-δ protein.

BiowMW :~ 135 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Diacylglycerol kinase delta; DAG kinase delta; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta; DGKD; KIAA0145; DGKδ; DGK δ

Immunogen :Synthetic peptide, corresponding to amino acids 34-88 of Human DGK-δ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of DGK-δ (S66) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:3T3-L1 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of DGK-δ (S66) pAb in paraffin-embedded human Rectum carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.