产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

FEN1 (A119) polyclonal antibody（KU）

货号: BS1989

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1989

Product Name :FEN1 (A119) polyclonal antibody（KU）

Swiss-Prot :P39748

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5' flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :FEN1 (A119) polyclonal antibody detects endogenous levels of FEN1 protein.

BiowMW :~ 42 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Flap endonuclease 1; FEN-1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1; MF1; hFEN-1; FEN1; RAD2

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human FEN1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of FEN1 (A119) pAb at 1:500 dilution Lane1:SP2/0 whole cell lysate(20ug) Lane2:C6 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of FEN1 (A119) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.