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GRK5 (Q382) polyclonal antibody（KU）

货号: BS1981

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1981

Product Name :GRK5 (Q382) polyclonal antibody（KU）

Swiss-Prot :P34947

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of b-adrenergic receptor kinase: GRK 2 (bARK, bARK1) and GRK 3 (bARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GRK5 (Q382) polyclonal antibody detects endogenous levels of GRK5 protein.

BiowMW :~ 68 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5

Immunogen :Synthetic peptide, corresponding to amino acids 350-400 of Human GRK5.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of GRK5 (Q382) pAb at 1:1000 dilution Lane1:PC3 whole cell lysate(20ug) Lane2:HEK293T whole cell lysate(20ug) Lane3:H9C2 whole cell lysate(20ug) Lane4:MEF whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of GRK5 (Q382) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of GRK5 (Q382) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.