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GRK 2 (D635) polyclonal antibody（KU）

货号: BS1963

价格：￥ 660

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1963

Product Name :GRK 2 (D635) polyclonal antibody（KU）

Swiss-Prot :P25098

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Background :Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of β-adrenergic receptor kinase, GRK 2 (βARK, βARK1) and GRK 3 (βARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GRK 2 (D635) polyclonal antibody detects endogenous levels of GRK 2 protein.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Beta-adrenergic receptor kinase 1; Beta-ARK-1; β-ARK-1; ARK-β1; G-protein coupled receptor kinase 2; ADRBK1; BARK; BARK1; GRK2; ARK β1; ARKβ1; β-ARK1

Immunogen :Synthetic peptide, corresponding to amino acids 600-650 of Human GRK2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of GRK2 (D635) pAb at 1:500 dilution Lane1:MEF whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug) Lane5:K562 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of GRK 2 (D635) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.