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HMGB1 (A164) polyclonal antibody（KU）

货号: BS1918

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1918

Product Name :HMGB1 (A164) polyclonal antibody（KU）

Swiss-Prot :P09429

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Research suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture, including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, ATF-2, c-Jun and members of the NFκB family to activate transcription. Additional studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG-1 and HMG-2 each contain two DNA-binding domains, termed HMG boxes. HMG proteins bind singlestranded DNA but induce conformational changes in double-stranded DNA alone.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HMGB1 (A164) polyclonal antibody detects endogenous levels of HMGB1 protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1

Immunogen :Synthetic peptide, corresponding to amino acids 131-180 of Human HMGB1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of HMGB1 (A164) polyclonal antibody at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:HL-60 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of HMGB1 (A164) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of HMGB1 (A164) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Apaf-1 Pyroptosome Senses Mitochondrial Permeability Transition

JournalCell Metabolism

ApplicationWB

IF21.567

SpeciesMouse

PMID
Qingchang Wenzhong Decoction Prevents the Occurrence of Intestinal Tumors by Regulating Intestinal Microbiota and Gasdermin E

JournalFrontiers in Physiology

ApplicationIHC

IF4.755

SpeciesMouse

PMID35910578
Bile acids target mitofusin 2 to differentially regulate innate immunity in physiological versus cholestatic conditions

JournalCell Reports

ApplicationWB

IF9.995

SpeciesHuman,Mouse

PMID36656708