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AIRE (P126) polyclonal antibody（KU）

货号: BS1879

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS1879

Product Name :AIRE (P126) polyclonal antibody（KU）

Swiss-Prot :O43918

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The autoimmune regulator gene, which is defective in the hereditary autoimmune disease APECED, encodes the transcriptional activator AIRE. AIRE is expressed in the medullary epithelial cells and monocyte-dendritic cells of the thymus, with lower expression in the spleen, fetal liver and lymph nodes. In adult tissue, AIRE expression in the thymus is confined to the medulla and the cortico-medullary junction, where it is modulated by thymocytes undergoing negative selection. At the cellular level, AIRE is located in microtubular structures of the cytoskeleton and in discrete nuclear dots resembling ND10 nuclear bodies. AIRE is induced by developing early thymocytes and is associated with the correct establishment of a regular thymic environment. AIRE regulates thymic architecture via transcriptional control of downstream target genes. AIRE mutations in APECED patients may affect thymic T cell selection and the formation of self-tolerance.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AIRE (P126) polyclonal antibody detects endogenous levels of AIRE protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Autoimmune regulator; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; APECED protein; AIRE; APECED; AIRE1; AIRE-1; APECED protein homolog

Immunogen :Synthetic peptide, corresponding to amino acids 101-150 of Human AIRE.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AIRE (P126) pAb at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:The Heart tissue lysate of Rat(40ug) Lane4:The Heart tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of AIRE (P126) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of AIRE (P126) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.