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IKKα/β (Q174) polyclonal antibody

货号: BS1756

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1756

Product Name :IKKα/β (Q174) polyclonal antibody

Swiss-Prot :O15111/O14920

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IKKα/β (Q174) polyclonal antibody detects endogenous levels of IKKα/β protein.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,PI3K AKT signaling,Immune Cell Signaling,STING Signaling,Inhibition of Apoptosis,

Alternative Name :I kappa-B kinase alpha; IkBKA; IKK-alpha; IKK-A; IkappaB kinase; EC 2.7.11.10; I-kappa-B kinase 1; IKK1; Conserved helix-loop-helix ubiquitous kinase; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; CHUK; IKKA; TCF16

Immunogen :Synthetic peptide, corresponding to amino acids 151-200 of Human IKKα

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IKKα /β (Q174) polyclonal antibody at 1:500 dilution Lane1:COS-7 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug) Lane6:CT26 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of IKKα/β (Q174) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation

JournalCancer Cell International

ApplicationWB

IF3.439

SpeciesHuman

PMID31572058
Mitoxantrone attenuates lipopolysaccharide-induced acute lung injury via inhibition of NEDD8 activating enzyme

Journal10.1016/j.intimp.2024.113605

ApplicationWB

IF4.8

SpeciesMouse

PMID39541844