产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Cyclin D1 (L283) polyclonal antibody（KU）

货号: BS1741

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1741

Product Name :Cyclin D1 (L283) polyclonal antibody（KU）

Swiss-Prot :P24385

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cyclin D1 (L283) polyclonal antibody detects endogenous levels of Cyclin D1 protein.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1

Immunogen :Synthetic peptide, corresponding to amino acids 250-300 of Human Cyclin D1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cyclin D1 (L283) pAb at 1:2000 dilution Lane1:HepG2 whole cell lysate(20ug) Lane2:L02 whole cell lysate(20ug) Lane3:C6 whole cell lysate(20ug) Lane4:3T3-L1 whole cell lysate(20ug)

Immunohistochemistry (IHC) analyzes of Cyclin D1 (L283) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

MicroRNA miR-302 inhibits the tumorigenicity of endometrial cancer cells by suppression of Cyclin D1 and CDK1

JournalCancer Letters

ApplicationWB

IF6.508

SpeciesHuman

PMID
A novel small-molecule compound diaporine A inhibits non-small cell lung cancer growth by regulating miR-99a/mTOR signaling

JournalCancer Biology & Therapy

ApplicationWB

IF4.742

SpeciesHuman

PMID25046358
Reconstructed mung bean trypsin inhibitor targeting cell surface GRP78 induces apoptosis and inhibits tumor growth in colorectal cancer

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB

IF3.673

SpeciesHuman,Mouse

PMID
MicroRNA-34a Suppresses Cell Proliferation by Targeting LMTK3 in Human Breast Cancer MCF-7 Cell Line

JournalDna And Cell Biology

ApplicationWB

IF3.311

SpeciesHuman

PMID
MicroRNA-99a induces G1-phase cell cycle arrest and suppresses tumorigenicity in renal cell carcinoma

JournalBmc Cancer

ApplicationWB

IF2.933

SpeciesMouse

PMID