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EGFR (V1010) polyclonal antibody（KU）

货号: BS1533

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1533

Product Name :EGFR (V1010) polyclonal antibody（KU）

Swiss-Prot :P00533

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :EGFR (V1010) polyclonal antibody detects endogenous levels of EGFR protein.

BiowMW :~ 175 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,

Alternative Name :Epidermal growth factor receptor; EC=2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR

Immunogen :Synthetic peptide, corresponding to amino acids 981-1030 of Human EGFR.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of EGFR (V1010) pAb at 1:500 dilution Lane1:HCC827 whole cell lysate(20ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:The Lung tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of EGFR (V1010) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

High expression of GPER1, EGFR and CXCR1 is associated with lymph node metastasis in papillary thyroid carcinoma

JournalInternational Journal of Clinical and Experimental Pathology

ApplicationIHC

IF0.205

SpeciesHuman

PMID25031742
Concomitant high expression of ERα36, EGFR and HER2 is associated with aggressive behaviors of papillary thyroid carcinomas

JournalScientific Reports

ApplicationIHC

IF3.998

SpeciesHuman

PMID28947799
Role of GPER1, EGFR and CXCR1 in differentiating between malignant follicular thyroid carcinoma and benign follicular thyroid adenoma

JournalInternational Journal of Clinical and Experimental Pathology

ApplicationIHC

IF0.205

SpeciesHuman

PMID26617848