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Actin-α/γ (Q49) polyclonal antibody

货号: BS1500

价格：￥ 660

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1500

Product Name :Actin-α/γ (Q49) polyclonal antibody

Swiss-Prot :P68032/P63261/P63267/P68133

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90%".

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Actin-α/γ (Q49) polyclonal antibody detects endogenous levels of Actin-α/γ protein.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,

Alternative Name :Actin; alpha cardiac muscle 1; Alpha-cardiac actin; ACTC1; ACTC

Immunogen :Synthetic peptide, corresponding to the N-terminal of Human Actin-α.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of Actin-α/γ (Q49) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing Cytoplasm and Cytoskeletal staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.