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Vimentin (I444) polyclonal antibody（KU）

货号: BS1491

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1491

Product Name :Vimentin (I444) polyclonal antibody（KU）

Swiss-Prot :P08670

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Vimentin (I444) polyclonal antibody detects endogenous levels of Vimentin protein.

BiowMW :~ 40, 57 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :Vimentin; VIM

Immunogen :Synthetic peptide, corresponding to amino acids 411-460 of Human Vimentin.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry (IHC) analyzes of Vimentin (I444) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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ApplicationIF

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SpeciesMouse

PMID39895262
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