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GAD-65/67 (D572) polyclonal antibody

货号: BS1400

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1400

Product Name :GAD-65/67 (D572) polyclonal antibody

Swiss-Prot :Q99259/Q05329

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GAD-65/67 (D572) polyclonal antibody detects endogenous levels of GAD65 and GAD67 protein.

BiowMW :~ 65, 67 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD 65; Glutamate decarboxylase 65 kDa isoform; GAD2; GAD-65; GAD 2; GAD-2; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD 67; Glutamate decarboxylase 67 kDa isoform; GAD1; GAD; GAD67; GAD-1; GAD 1

Immunogen :Synthetic peptide, corresponding to amino acids 540-590 of Human GAD-65.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of GAD-65/67 (D572) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of GAD-65/67 (D572) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.