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Cyclin B1 (I120) polyclonal antibody（KU）

货号: BS1392

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1392

Product Name :Cyclin B1 (I120) polyclonal antibody（KU）

Swiss-Prot :P14635

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13 SUC1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has estalished that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cyclin B1 (I120) polyclonal antibody detects endogenous levels of Cyclin B1 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :CCNB1; CCNB; cyclin B1; G2/mitotic-specific cyclin-B1

Immunogen :Synthetic peptide, corresponding to amino acids 90-140 of Human Cyclin B1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cyclin B1 (I120) polyclonal antibody at 1:500 dilution Lane1:MCF-7whole cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate

Immunohistochemistry (IHC) analyzes of Cyclin B1 (I120) pAb in paraffin-embedded human breast carcinoma tissue.

WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics

JournalInternational Journal of Biological Sciences

ApplicationWB

IF4.067

SpeciesHuman

PMID29989053
Fangchinoline derivatives inhibits PI3K signaling in vitro and in vivo in non-small cell lung cancer

JournalBIOORGANIC CHEMISTRY

ApplicationWB

IF5.307

SpeciesHuman

PMID37295240
The molecular mechanism of action for the potent antitumor component extracted using supercritical fluid extraction from Croton crassifolius root

JournalJOURNAL OF ETHNOPHARMACOLOGY

ApplicationWB

IF5.4

SpeciesHuman

PMID38490290