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ACCα (K86) polyclonal antibody（KU）

货号: BS1377

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS1377

Product Name :ACCα (K86) polyclonal antibody（KU）

Swiss-Prot :Q13085

Host :Rabbit

Reactivity :Human,Mouse,Rat,Pig

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACCα (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCβ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCα is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACCα, which maps to human chromosome 17, encodes the 265 kDa α form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCβ is homologous to that of ACCα except for an additional peptide of about 150 amino acids at the N-terminus.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ACCα (H74) polyclonal antibody detects endogenous levels of ACCα protein.

BiowMW :~ 265 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect,AMPK Signaling Pathway,

Alternative Name :Acetyl-CoA carboxylase 1; Short name=ACC1; ACC-alpha; Biotin carboxylase; ACACA; ACAC; ACC1; ACCA

Immunogen :Synthetic peptide, corresponding to amino acids 50-100 of Human ACCα.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ACCα (K86) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Jurkat cell lysate Lane3:NIH-3T3 cell lysate Lane4:PC12 cell lysate

Immunohistochemistry (IHC) analyzes of ACCα (K86) pAb in paraffin-embeddedhuman stomach carcinoma tissue at 1:50.showing cell membrane, cytoplasmicstaining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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