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HSP90α (P717) polyclonal antibody（KU）

货号: BS1180

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1180

Product Name :HSP90α (P717) polyclonal antibody（KU）

Swiss-Prot :P07900

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Background :In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HSP90α (P717) polyclonal antibody detects endogenous levels of HSP90α protein.

BiowMW :~ 85, 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Nuclear Receptor Signaling,Regulation of Actin Dynamics,

Alternative Name :Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; HSP90AA1; HSP90A; HSPC1; HSPCA

Immunogen :Synthetic peptide, corresponding to amino acids 671-720 of Human HSP90α.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of HSP90α (P717) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:L02 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of HSP90α (P717) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 cell lysate using HSP90α (P717) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HSP90α (P717) #BS1180.“↑”（supernatant）; “↓(deposition）

Lanosterol modulates proteostasis via dissolving cytosolic sequestosomes/aggresome-like induced structures

JournalBiochimica Et Biophysica Acta-Molecular Cell Research

ApplicationWB

IF4.105

SpeciesHuman

PMID31785334