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HSP60 (T547) polyclonal antibody（KU）

货号: BS1179

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1179

Product Name :HSP60 (T547) polyclonal antibody（KU）

Swiss-Prot :P10809

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Background :HSPs (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins, and the HSP 110 family. The constitutively expressed mitochondrial protein HSP 60 shares the ability to recognize and stabilize proteins during folding, assembly and disassembly with other HSP family members. The mitochondrial and cytosolic localization of HSP 60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker forr γ/δ T cell recognition.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HSP60 (T547) polyclonal antibody detects endogenous levels of HSP60 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Mitochondrial Control of Apoptosis,

Alternative Name :60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; HSPD1; HSP60

Immunogen :Synthetic peptide, corresponding to amino acids 511-560 of Human HSP60.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of HSP60 (T547) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of HSP60 (T547) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 cell lysate using HSP60 (T547) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HSP60 (T547) pAb #BS1179.“↑”（supernatant）; “↓”（deposition）

Curcumin induces mitophagy by promoting mitochondrial succinate dehydrogenase activity and sensitizes human papillary thyroid carcinoma BCPAP cells to radioiodine treatment

JournalTOXICOLOGY IN VITRO

ApplicationWB

IF3.2

SpeciesHuman

PMID37634662