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HDAC8 (K33) polyclonal antibody（KU）

货号: BS1167

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1167

Product Name :HDAC8 (K33) polyclonal antibody（KU）

Swiss-Prot :Q9BY41

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Background :In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HDAC8 (K33) polyclonal antibody detects endogenous levels of HDAC8 protein.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,

Alternative Name :Histone deacetylase 8; HD8; HDAC8; HDACL1; CDA07

Immunogen :Synthetic peptide, corresponding to amino acids 2-45 of Human HDAC8.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of HDAC8 (K33) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:L02 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of HDAC8 (K33) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 cell lysate using HDAC8 (K33) pAb (Sepharose Bead Conjugate) #BD0046(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HDAC8 (K33) pAb #BS1167.“↑”（supernatant）; “↓”（deposition）