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HDAC1 (E468) polyclonal antibody（KU）

货号: BS1160

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1160

Product Name :HDAC1 (E468) polyclonal antibody（KU）

Swiss-Prot :Q13547

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Background :Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HDAC1(E468) polyclonal antibody detects endogenous levels of HDAC1 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Notch Signaling,Nuclear Receptor Signaling,Cell Cycle Control G1 S Checkpoint,DNA Methylation,Epigenetic Writers and Erasers of Histone H3,Epigenetic Writers and Erasers of Histones H2A H2B and H4,

Alternative Name :Histone deacetylase 1; HD1; RPD3L1

Immunogen :Synthetic peptide, corresponding to amino acids 435-486 of Human HDAC1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of HDAC1 (E468) polyclonal antibody at 1:500 dillution Lane1:HEK293T whole cell lysate(40μg) Lane2:Hela whole cell lysate(40μg) Lane3:The Kidney tissue lysate of Mouse(40μg) Lane4:The Kidney tissue lysate of Rat(40μg) Lane5:The Heart tissue lysate of Mouse(40μg) Lane6:The Heart tissue lysate of Rat(40μg)

Immunohistochemistry (IHC) analyzes of HDAC1 (E468) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 cell lysate using HDAC1 (E468) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HDAC1 (E468) pAb #BS1160.“↑”（supernatant）; “↓”（deposition）