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GSK3β (S13) polyclonal antibody（KU）

货号: BS1157

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1157

Product Name :GSK3β (S13) polyclonal antibody（KU）

Swiss-Prot :P49841

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF IP

Application_all :WB: 1:500~1:1000 IF: 1:50~200 IP: 1:50~1:200

Background :Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :GSK3β (S13) polyclonal antibody detects endogenous levels of GSK3β protein.

BiowMW :~ 46 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Hedgehog Signaling,Hypoxia Signaling,Translational Contral Regulation of elF2,Regulation of Microtubule Dynamics,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,MTOR signaling,

Alternative Name :Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

Immunogen :Synthetic peptide, corresponding to Human GSK3β.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of GSK3β (S13) polyclonal antibody at 1:1000 dilution Lane1:The Spleen tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug)

Immunofluorescence analysis of MCF-7 cells using GSK3β (S13) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of HepG2 cell lysates using GSK3β pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using GSK3β pAb.

Chronic noise exposure causes persistence of tau hyperphosphorylation and formation of NFT tau in the rat hippocampus and prefrontal cortex

JournalExperimental Neurology

ApplicationWB

IF4.691

SpeciesRat

PMID22971273
LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells

JournalMolecular Carcinogenesis

ApplicationWB

IF3.411

SpeciesHuman

PMID25945460