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eEF2K (P360) polyclonal antibody（KU）

货号: BS1100

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1100

Product Name :eEF2K (P360) polyclonal antibody（KU）

Swiss-Prot :O00418

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :eEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :eEF2K (P360) polyclonal antibody detects endogenous levels of eEF2K protein.

BiowMW :~ 105 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :Elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K

Immunogen :Synthetic peptide, corresponding to amino acids 331-380 of Human eEF2K.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of eEF2K (P360) polyclonal antibody at 1:1000 dillution Lane1:Hela whole cell lysate(40μg) Lane2:HCT-116 whole cell lysate(40μg) Lane3:C6 whole cell lysate(60μg)

Immunohistochemistry (IHC) analyzes of eEF2K (P360) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.