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Cyclin G (I194) polyclonal antibody（KU）

货号: BS1088

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1088

Product Name :Cyclin G (I194) polyclonal antibody（KU）

Swiss-Prot :P51959

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. The Cdc2- cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression. The G1 to S transition, however, appears to be controlled by the G1 cyclins. Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cyclin G(I194) polyclonal antibody detects endogenous levels of Cyclin G protein.

BiowMW :~ 29 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cyclin-G1; Cyclin-G; CCNG1; CCNG; CYCG1

Immunogen :Synthetic peptide, corresponding to amino acids 180-220 of Human Cyclin G.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cyclin G (I194) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:SK-OVCAR3 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Cyclin G (I194)pAb in paraffin-embedded human breast carcinoma tissue at 1:100.