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Cyclin A (W250) polyclonal antibody（KU）

货号: BS1083

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1083

Product Name :Cyclin A (W250) polyclonal antibody（KU）

Swiss-Prot :P78396/P20248

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cyclin A (W250) polyclonal antibody detects endogenous levels of Cyclin A protein.

BiowMW :~ 52 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cyclin-A1; CCNA1; Cyclin A1; CyclinA1; Cyclin-A2; Cyclin-A; Cyclin A; CyclinA; CCNA2; CCN1; CCNA

Immunogen :Synthetic peptide, corresponding to amino acids 220-270 of Human Cyclin A.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cyclin A (W250) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:The Testis tissue lysate of Mouse(40ug) Lane4:The Testis tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of Cyclin A (W250) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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