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ARC (E194) polyclonal antibody

货号: BS1018

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS1018

Product Name :ARC (E194) polyclonal antibody

Swiss-Prot :O60936

Host :Rabbit

Reactivity :Human

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Growth factor stimulation has been shown to induce the expression of immediate early genes in non-neuronal cells, which encode a variety of molecules that are potentially involved in long-term cellular responses. Similar responses induced by neurotransmitter stimulation have also been seen in neuronal cells and evidence suggests that protein synthesis is required for long-term synaptic plasticity. Arc (for activity-regulated cytoskeleton-associated protein) is a growth factor and immediate early gene that is enriched in brain. Arc mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for Arc in activitydependent changes in dendrite function. Arc expression has been detected in neuronal cell bodies and dendrites in the hippocampus, amygdala, hypothalamus, striatum and cortex. Arc has been shown to localize to the cytoskeleton of neuronal cells and appears to co-localize with F-Actin, although it may associate with an Actin-associated protein rather than directly with F-Actin. It has been shown that cocaine-stimulated neuronal activity results in increased Arc mRNA levels in striatum.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ARC (E194) polyclonal antibody detects endogenous levels of ARC protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nucleolar protein 3, Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Myp, Nucleolar protein of 30 kDa, Nop30, NOL3, ARC, NOP

Immunogen :Synthetic peptide, corresponding to amino acids 161-210 of Human ARC.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ARC (E194) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(30ug) Lane3:L02 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ARC (E194) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesHuman

PMID27573706