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Apaf-1-ALT (H324) polyclonal antibody（KU）

货号: BS1016

价格：￥ 660

货期：现货（1-2个工作日）

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No. :BS1016

Product Name :Apaf-1-ALT (H324) polyclonal antibody（KU）

Swiss-Prot :O14727-6

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :APAF-1-ALT harbors the caspase recruitment domain and an incomplete CED-4 like/ATPase domain, but lacks the WD-40 repeat units. The LNCaP cell expressed the full-length APAF-1 weakly and APAF-1-ALT rather abundantly, especially after mycoplasma infection. LNCaP underwent a retarded DNA damage-induced apoptosis, which was independent of caspase 9 activity. APAF-1-ALT functioned less effectively in inducing apoptosis than did APAF-1-XL, the full-length APAF-1, in transient transfection. Moreover, APAF-1-ALT interfered with APAF-1-XL's ability to induce apoptosis in transient double transfection experiment. These results indicate that APAF-1-ALT is a molecule that is deficient and impeded for mediating apoptosis and that it may contribute to the resistance to DNA damage-induced treatment observed in LNCaP.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :APAF-1-ALT(H324) polyclonal antibody detects endogenous levels of APAF-1 Isoform 6 (Apaf-1-ALT) protein.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Apaf-1 Isoform 6; APAF1 Isoform 6;

Immunogen :Synthetic peptide, corresponding to amino acids 301-350 of Human Apaf-1-ALT.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of APAF-1-ALT (H324) polyclonal antibody in extracts from COLO205 cells.

Immunohistochemistry (IHC) analyzes of Apaf-1-ALT (H324) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

MicroRNA-145 Protects Cardiomyocytes against Hydrogen Peroxide (H2O2)-Induced Apoptosis through Targeting the Mitochondria Apoptotic Pathway

JournalPLoS One

ApplicationWB

IF2.74

SpeciesRat

PMID23028672
Alteration of apoptotic protease activating factor 1 expression and possible role in ONOO--induced apoptosis in human cerebral vascular smooth muscle cells

JournalInternational Journal of Clinical and Experimental Medicine

ApplicationWB

IF0.166

SpeciesHuman

PMID26770639