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AhR (K32) polyclonal antibody（KU）

货号: AP0420

价格：￥ 660

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0420

Product Name :AhR (K32) polyclonal antibody（KU）

Swiss-Prot :P35869

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :AhR (K32) polyclonal antibody detects endogenous levels of AhR protein.

BiowMW :~ 94 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76

Immunogen :Synthetic peptide, corresponding to amino acids N-terminus of Human AhR.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of AhR (K32) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate

Immunohistochemistry (IHC) analyzes of AhR (K32) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of AhR (K32) pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.