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Cyclin D3 (G277) polyclonal antibody

货号: AP0379

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :AP0379

Product Name :Cyclin D3 (G277) polyclonal antibody

Swiss-Prot :P30281

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation.Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cyclin D3 (G277) polyclonal antibody detects endogenous levels of Cyclin D3 protein.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CCND 3; CCND3; CyclinD3; Cyclin-D3; D3 type cyclin; G1 S specific cyclin D3; G1/S specific cyclin D3; G1/S-specific cyclin-D3;

Immunogen :Synthetic peptide, corresponding to amino acids 249-299 of Human Cyclin D3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cyclin D3 (G277) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate