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HSL (P548) polyclonal antibody

货号: AP0372

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0372

Product Name :HSL (P548) polyclonal antibody

Swiss-Prot :Q05469

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000

Background :HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HSL (P548) polyclonal antibody detects endogenous levels of HSL protein.

BiowMW :~ 85, 115 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Insulin &Glucose Signaling,

Alternative Name :Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; Hormone-sensitive lipase; HSL; LHS; Lipase hormone sensitive; LIPE; LIPS;

Immunogen :Synthetic peptide, corresponding to amino acids 520-570 of Human HSL.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of HSL (P548) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate

Immunohistochemistry (IHC) analyzes of HSL (P548) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.

Mark4 promotes adipogenesis and triggers apoptosis in 3T3-L1 adipocytes by activating JNK1 and inhibiting p38MAPK pathways

JournalBiology Of The Cell

ApplicationWB

IF4.458

SpeciesMouse

PMID
The local corticotropin-releasing hormone receptor 2 signalling pathway partly mediates hypoxia-induced increases in lipolysis via the cAMP–protein kinase A signalling pathway in white adipose tissue

JournalMolecular And Cellular Endocrinology

ApplicationWB

IF3.871

SpeciesRat

PMID
αMSH promotes preadipocyte proliferation by alleviating ER stress-induced leptin resistance and by activating Notch1 signal in mice

JournalBiochimica Et Biophysica Acta-Molecular Basis Of Disease

ApplicationWB

IF4.352

SpeciesMouse

PMID27717825
miR-103 promotes 3T3-L1 cell adipogenesis through AKT/mTOR signal pathway with its target being MEF2D

JournalBiological Chemistry

ApplicationWB

IF3.915

SpeciesMouse

PMID25400071
Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway

JournalDiabetes Metabolic Syndrome and Obesity-Targets and Therapy

ApplicationWB

IF2.842

SpeciesMouse

PMID31564937