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Stat3 (S727) polyclonal antibody（KU）

货号: AP0366

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0366

Product Name :Stat3 (S727) polyclonal antibody（KU）

Swiss-Prot :P40763

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF IP

Application_all :WB:1:500~1:1000 IF: 1:50~1:200 IP: 1:50~1:200

Background :Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Stat3 (S727) polyclonal antibody detects endogenous levels of Stat3 protein.

BiowMW :~ 88 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

Immunogen :Synthetic peptide, corresponding to Human Stat3.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Stat3 (S727) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:PC3 whole cell lysate(40μg) Lane3:AML-12 whole cell lysate(40μg) Lane4:C6 whole cell lysate(40μg)

Immunofluorescence analysis of U-87MG cells using Stat3 antibody at dilution of 1:50.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody. Western blot was performed from the immunoprecipitate using L3MBTL3 antibody at a dilition of 1:1000.

Immunoprecipitation of Hela cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb.

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