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eIF4B (phospho-Ser422) polyclonal antibody

货号: AP0084

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :AP0084

Product Name :eIF4B (phospho-Ser422) polyclonal antibody

Swiss-Prot :P23588

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-eIF4B(Ser422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B

Immunogen :Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-eIF4B (Ser422) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate