产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ERBB2 polyclonal antibody

货号: BS1170

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1170

Product Name :ERBB2 polyclonal antibody

Swiss-Prot :P04626

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity. While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members. Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers. Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase. ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ERBB2 polyclonal antibody detects endogenous levels of ERBB2 protein.

BiowMW :~ 190kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,ErbB HER Signaling,

Alternative Name :Receptor tyrosine-protein kinase erbB-2;Receptor tyrosine-protein kinase erbB-2;MLN 19;Proto-oncogene Neu;Proto-oncogene c-ErbB-2;Tyrosine kinase-type cell surface receptor HER2;p185erbB2;CD340;ERBB2

Immunogen :Synthetic peptide, corresponding to Human ERBB2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ERBB2 polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug)