Product Name :β-Actin (4D3) monoclonal antibody-HRP
Reactivity :H,M,R(not applicable to mouse tissue)
Application_all :WB: 1:5000~1:20000
Background :Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :β-Actin (4D3) detects endogenous levels of β-actin protein.
Figure1 :Western blot (WB) analysis of β-Actin (4D3) mAb-HRP at 1:2000/1:5000/1:10000 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:NIH-3T3 whole cell lysate(40ug)
Note :For research use only, not for use in diagnostic procedure.
Pathway :Contribution of ECM and Cytoskeletal Factors to EMT,Adherens Junction signaling,
Alternative Name :Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β; Beta actin;
Immunogen :Recombinant full length Human β-Actin.
Modification :Unmodification
