Product Name :GAPDH Rabbit monoclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:10000~1:20000
IHC:1:500~1:1000
IF:1:500~1:1000
Background :Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels.
Product :0.5mg/ml in PBS with 0.05% proclin300, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :GAPDH mAb detects endogenous levels of GAPDH protein.
Figure1 :Western blot (WB) analysis of GAPDH Rabbit mAb at 1:10000 dilution
Lane1:A549 whole cell lysate(20ug)
Lane2:The Heart tissue lysate of Mouse(20ug)
Lane3:The Spleen tissue lysate of Mouse(20ug)
Lane4:The Lung tissue lysate of Rat(20ug)
Lane5:The Kidney tissue lysate of Rat(20ug)
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD
Immunogen :Recombinant full length Human GAPDH.
