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CTCF polyclonal antibody

货号: BS79643

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79643

Product Name :CTCF polyclonal antibody

Swiss-Prot :P49711

Host :Rabbit

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB, IHC, IF/ICC, IP, ChIP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:100|ChIP,1:50 - 1:200

Background :This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :140KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CTCF;MRD21;CTCF

Immunogen :Recombinant fusion protein of human CTCF(NP_006556.1).

Conjugate :Unconjugated

Modification :Unmodification

Immunofluorescence analysis of NIH/3T3 cells using CTCF Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using CTCF Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using CTCF Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CTCF antibody . Western blot was performed from the immunoprecipitate using CTCF antibody at a dilution of 1:500.