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Akt2 polyclonal antibody

货号: BS6473

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6473

Product Name :Akt2 polyclonal antibody

Swiss-Prot :P31751

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF

Application_all :WB,1:500 - 1:1000|IHC,1:100 - 1:200|IF/ICC,1:100 - 1:500

Background :This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AKT2;HIHGHH;PKBB;PKBBETA;PRKBB;RAC-BETA

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human AKT2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of AKT2 expression in A549 (A), LO2 (B), AML12 (C), C6 (D) whole cell lysates.

Immunohistochemical analysis of AKT2 staining in human brain formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of AKT2 staining in HepG2 cells.

miRNA-302b Suppresses Human Hepatocellular Carcinoma by Targeting AKT2

JournalMolecular Cancer Research

ApplicationWB

IF5.852

SpeciesHuman

PMID24337067
MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells

JournalBmc Cancer

ApplicationWB

IF4.43

SpeciesHuman

PMID24083596
MiR-200c regulates tumor growth and chemosensitivity to cisplatin in osteosarcoma by targeting AKT2

JournalScientific Reports

ApplicationWB

IF4.379

SpeciesMouse,Human

PMID29051585
High Uric Acid Induces Insulin Resistance in Cardiomyocytes In Vitro and In Vivo

JournalPLoS One

ApplicationWB

IF3.24

SpeciesRat

PMID26836389
GPR30-mediated HMGB1 upregulation in CAFs induces autophagy and tamoxifen resistance in ERα-positive breast cancer cells

JournalAging-US

ApplicationWB

IF4.831

SpeciesHuman

PMID34182538