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Trk B (phospho-Y706) polyclonal antibody（KU）

货号: BS4201

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4201

Product Name :Trk B (phospho-Y706) polyclonal antibody（KU）

Swiss-Prot :Q16620

Host :Rabbit

Reactivity :Human

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B, and is a functional receptor for NT-3.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Trk B (Y706) polyclonal antibody detects endogenous levels of Trk B protein only when phosphorylated at Tyr706

BiowMW :~ 92, 145 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; NTRK2; TRKB

Immunogen :Synthetic phosphopeptide derived from human Trk B around the phosphorylation site of Tyrosine 706.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Trk B (phospho-Y706) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,5 minutes) whole cell lysate(40ug) Lane3:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Trk B (Y706) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Sigma 1 receptor activation regulates brain-derived neurotrophic factor through NR2A-CaMKIV-TORC1 pathway to rescue the impairment of learning and memory induced by brain ischaemia/reperfusion

JournalPsychopharmacology

ApplicationWB

IF3.13

SpeciesMouse

PMID25420607