Product Name :IRAK-M (L519) polyclonal antibody
Application_all :IHC: 1:50~1:200
IF: 1:50~1:200
Background :Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. A 100 kDa IL-1- dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFκB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFκB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :IRAK-M (L519) polyclonal antibody detects endogenous levels of IRAK-M protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Toll-like Receptor signaling,
Alternative Name :Interleukin-1 receptor-associated kinase 3; IRAK-3; IL-1 receptor-associated kinase M; IRAKM; IRAK3
Immunogen :Synthetic peptide, corresponding to amino acids 500-540 of Human IRAK-M.
Modification :Unmodification
