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mTOR (S2442) polyclonal antibody（KU）

货号: BS1844

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1844

Product Name :mTOR (S2442) polyclonal antibody（KU）

Swiss-Prot :P42345

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :mTOR (S2442) polyclonal antibody detects endogenous levels of mTOR protein.

BiowMW :~ 289 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Immunogen :Synthetic peptide, corresponding to amino acids 2430-2480 of Human mTOR.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of mTOR (S2442) at 1:500 dillution Lane1:NIH-3T3 whole cell lysate(40μg) Lane2:HEK293T whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:MCF-7 whole cell lysate(40μg) Lane6:HepG2 whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of mTOR (S2442) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Immunoprecipitation of SGC7901 cell lysates using mTOR (S2442) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using mTOR (S2442) pAb #BS1844.“↑”(supernatant); “↓”(deposition).

SIRT3 Protects Rotenone-induced Injury in SH-SY5Y Cells by Promoting Autophagy through the LKB1-AMPK-mTOR Pathway

JournalAging and Disease

ApplicationIHC

IF4.232

SpeciesHuman

PMID29896416
Low-dose radiation exaggerates HFD-induced metabolic dysfunction by gut microbiota through PA-PYCR1 axis

JournalCommunications Biology

ApplicationWB，IHC

IF6.548

SpeciesHuman,Mouse

PMID36088469
Potential role of gut microbiota-LCA-INSR axis in high fat-diet-induced non-alcoholic fatty liver dysfunction: From perspective of radiation variation

JournalCurrent Research in Food Science

ApplicationWB

IF6.269

SpeciesHuman

PMID36204709